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. 2014 May 21;2014:935251. doi: 10.1155/2014/935251

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Copyright © 2014 Ping Cheng et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Effect of NaHS pretreatment on regulation of apoptosis in vivo. (a) The mRNA expression of Bcl-2 and Bax in liver tissues was detected by real time PCR. *P < 0.05 for saline versus saline + I/R, ^# P < 0.05 for saline + I/R versus I/R + NaHS (14 μmol/kg). (b) Western blots and quantitative evaluation of the expression of Bcl-2 and Bax in liver tissues. *P < 0.05 for saline versus saline + I/R,^# P < 0.05 for saline + I/R versus I/R + NaHS (14 μmol/kg). (c) Immunohistochemistry staining (200x) showed the expression of Bcl-2 and Bax in liver tissue at 12 h. *P < 0.05 for saline + I/R versus I/R + NaHS. (d) TUNNEL staining showed the apoptotic cells in three groups at 12 h. Original magnifications: ×200. *P < 0.05 for saline versus saline + I/R, ^# P < 0.05 for saline + I/R versus I/R + NaHS.