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. 2014 May 21;2014:403515. doi: 10.1155/2014/403515

Figure 2.

Figure 2

PRRSV infection increases IL-1β mRNA expression and secretion in PAMs. (a, b) PAMs were mock infected or infected with PRRSV at a MOI of 0.1. Cells and supernatants were collected separately at the indicated time points and subjected to real-time RT-PCR (a) and ELISA (b) to analyze the expression of IL-1β. (c, d) PAMs were infected with PRRSV at different doses (0.01, 0.1, 0.2, and 0.5 MOI). Cells and supernatants were collected separately at 36 h postinfection for analysis by IL-1β-specific real-time RT-PCR (c) and ELISA (d). The mock-infected cells were used as negative controls. *P < 0.05 and **P < 0.01 compared with the mock-infected cells.