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. 2014 May 21;2014:403515. doi: 10.1155/2014/403515

Figure 4.

Figure 4

PMB has no effect on PRRSV proliferation or PRRSV-induced IL-1β production. (a) PAMs were infected with the adapted PRRSV at a MOI of 0.1, followed by treatment with polymyxin B (0, 5, 10, and 20 μg/mL) in the absence of serum, for 36 h. Supernatants were collected at different time point (12, 24, 36, and 48 h) postinfection for plaque assay to determine viral titers. (b, c) PAMs were infected with the adapted PRRSV at a MOI of 0.1 or treated with LPS (1 μg/mL), followed by treatment with polymyxin B (0, 5, 10, and 20 μg/mL) in the absence of serum, for 36 h. The cells and supernatants were then harvested separately and analyzed by real-time RT-PCR and ELISA, respectively.