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. 2014 May 21;2014:403515. doi: 10.1155/2014/403515

Figure 7.

Figure 7

PRRSV-induced IL-1β secretion requires NLRP3 inflammasome. (a–c) PAMs were transfected with 80 nM of psiNegative, psiNLRP3, or psiASC, respectively. At 24 h posttransfection, cells were mock infected or infected with PRRSV at a MOI of 0.1. The supernatants and cells were harvested separately 36 h later and analyzed by IL-1β ELISA (a) and real-time RT-PCR (b), respectively. For Western blotting to detect pro-IL-1β, cells were harvested at 24 h postinfection by using polyclonal antibody against pro-IL-1β (c). (d) PAMs were mock infected or infected with PRRSV at a MOI of 0.1, followed by treatment with caspase 1 inhibitor (5, 10, 25, 50, and 100 μM) or DMSO vehicle in the absence of serum, for 36 h. The supernatants were then harvested and analyzed by IL-1β-specific ELISA. *P < 0.05 and **P < 0.01 compared with DMSO-treated cells plus PRRSV infection.