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. 2014 May 21;2014:403515. doi: 10.1155/2014/403515

Figure 8.

Figure 8

TLR4/MyD88/NF-κB signaling pathway is involved in PRRSV-induced expression of NLRP3 inflammasome components. (a, b) PAMs were transfected with 80 nM of psiNegative, psiTLR4, or psiMyD88, respectively. At 24 h posttransfection, cells were mock infected or infected with PRRSV at a MOI of 0.1. The cells and supernatants were harvested separately 36 h later and analyzed by NLRP3/ASC/caspase 1 specific real-time RT-PCR. (c–e) PAMs were mock infected or infected with PRSSV at a MOI of 0.1, followed by treatment with NF-κB inhibitor (1, 2, 5, and 10 μM) or DMSO vehicle in the absence of serum, for 36 h. The cells were then harvested to analyse the mRNA expression of NLRP3 (c), ASC (d), and caspase 1 (e), respectively, by real-time RT-PCR. *P < 0.05 and **P < 0.01 compared with DMSO-treated cells plus PRRSV infection.