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. 2014 Jun 12;9(6):e98863. doi: 10.1371/journal.pone.0098863

Figure 6. The effect of β1-BKCa subunit on cell surface expression of α-BKCa splice variants.

Figure 6

Co-transfection of β1-BKCa subunit has little apparent impact on cell surface expression of α-BKCa ZERO or STREX variants. (A) Representative Western blot showing levels of membrane (MF), and cytosolic (CF) fractions of α-BKCa protein in CHO-K1 cultured cells, transfected with either α-BKCa splice variants ZERO or STREX. (B) In the CHO-K1 cell culture system, α-BKCa STREX variant shows dominant cell surface expression when co-transfected with β1-BKCa. Results are shown for n = 5 separate experiments and are presented as mean ± SEM (*P<0.05, unpaired t-test). (C) Membrane to cytosolic ratio for the expression of ZERO or STREX proteins when co-transfected with β1-BKCa in cultured CHO-K1 cells. Results are shown for n = 5 separate experiments and are presented as mean ± SEM (* P = 0.05, unpaired t-test).