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. 2014 Jun 12;9(6):e98863. doi: 10.1371/journal.pone.0098863

Figure 7. Cell surface expression of β1-BKCa subunit in the presence and absence of α-BKCa splice variants.

Figure 7

Co-transfection of ZERO or STREX variants with β1-BKCa subunit enhances cell surface trafficking of β1-BKCa subunit. The top image in panel (A) represents a Western blot for membrane (MF) and cytosolic (CF) fractions of β1-BKCa protein in CHO-K1 cultured cells, co-transfected with either α-BKCa splice variants ZERO or STREX. The bottom Figure in panel (A) shows the membrane vs cytosolic expression of transfected β1-BKCa channel protein when co-transfected with either α-BKCa splice variants of ZERO or STREX. Results are shown for n = 5 separate experiments. The top image in panel (B) shows a representative Western blot of membrane (MF) and cytosolic (CF) fractions of β1-BKCa protein in CHO-K1 cultured cells, in the absence of either α-BKCa splice variants ZERO or STREX. The bottom Figure in panel (B) shows membrane vs cytosolic expression of β1-BKCa subunit in cultured CHO-K1 cells in the absence of the α-BKCa splice variants ZERO or STREX. Results are shown for n = 6 separate experiments and are presented as mean ± SEM (*P<0.05, unpaired t-test).