Table 1. Sequence of primers used for end-point and real-time PCR.
Accession number | Primer sequence | Amplicon length | Amplification efficiency | |
End-point PCR | ||||
α-BKCa | NM_031828 | F: TACTGCAAGGCCTGTCATGATG | 342 | |
R: TCATCAGCTTCGGGGATGTGTT | ||||
Real-time PCR | ||||
STREX | NM_031828 | F: TTTGATTGCGGACGTTCTGA | 77 | 2.063 |
R: TCTCTCAAGGGTGTCCACGTTAC | ||||
SS4 | AF_135265 | F: CAAGTTGCCTTTGGTATCAGTC | 131 | 2.013 |
R: GCTCTGTGTCAGGGTCATC | ||||
ZERO | NM_031828 | F:AAACAAGTAATTCCATCAAGCTGGTG | 137 | 2.006 |
R: CGTAAGTGCCTGGTTGTTTTGG | ||||
β-actin | NM_031144 | F: CCTCTATGCCAACACAGTGCTGTCT | 128 | 1.993 |
R: GCTCAGGAGGAGCAATGATCTTGA |
(F: forward primer, R: reverse primer).