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. 2014 Jun 12;9(6):e98863. doi: 10.1371/journal.pone.0098863

Table 1. Sequence of primers used for end-point and real-time PCR.

Accession number Primer sequence Amplicon length Amplification efficiency
End-point PCR
α-BKCa NM_031828 F: TACTGCAAGGCCTGTCATGATG 342
R: TCATCAGCTTCGGGGATGTGTT
Real-time PCR
STREX NM_031828 F: TTTGATTGCGGACGTTCTGA 77 2.063
R: TCTCTCAAGGGTGTCCACGTTAC
SS4 AF_135265 F: CAAGTTGCCTTTGGTATCAGTC 131 2.013
R: GCTCTGTGTCAGGGTCATC
ZERO NM_031828 F:AAACAAGTAATTCCATCAAGCTGGTG 137 2.006
R: CGTAAGTGCCTGGTTGTTTTGG
β-actin NM_031144 F: CCTCTATGCCAACACAGTGCTGTCT 128 1.993
R: GCTCAGGAGGAGCAATGATCTTGA

(F: forward primer, R: reverse primer).