Figure 5. qRT-PCR experiments verifying the up-regulation of genes following Imatinib treatment.

Comparative qRT-PCR analyses of genes identified by microarray analyses to be up-regulated in schistosome couples 48 h following Imatinib (50 µM) treatment. The selected genes encode a predicted egg-shell precursor protein (Smp-000430; [47]), a ribosomal protein S6 kinase (rib. protein S6 kinase; Smp_017900), paramyosin (Smp_129550; [73]), HSP70 (Smp_106930; [77], [78]), fs800-like (Smp_000270; [79]), titin (Smp_105020), p14 (Smp_131110; [80]), and the tyrosinase 1 (SmTYR1; Smp_050270; [81]). Log₂ratios (treated/control) are given for all biological replicates comparing the results of three independent biological replicates using qRT-PCR (dark gray-shaded columns; numbered 1–3) with the results of three independent microarray analyses (light grey-shaded and white columns; numbered 4–6).