Figure 2.
EEC proliferation assessed using an MTT assay. EECs were incubated with different intervention factors for the indicated durations (48–72 h). The groups were as follows: Control, EECs incubated with serum-free DMEM/F12; AngII, EECs incubated with 10−6 mol/l AngII and serum-free DMEM/F12; Ang-(1–7), EECs incubated with 10−5 mol/l Ang-(1–7) and serum-free DMEM/F12; AngII+Ang-(1–7), EECs incubated with 10−6 mol/l AngII and 10−5 mol/l Ang-(1–7) and serum-free DMEM/F12. Values are presented as the mean ± standard deviation (n=6). *P<0.05 vs. the control group and #P<0.05 vs. the AngII group. EEC, endometrial epithelium cell; DMEM/F12, Dulbecco’s modified Eagle’s medium/Nutrient Mixture F-12; Ang, angiotensin; A value, absorbance value.