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. 2014 Apr 9;9(6):2180–2186. doi: 10.3892/mmr.2014.2128

Figure 4.

Figure 4

Ang-(1–7) inhibits Ang II-induced EEC transdifferentiation. EECs were incubated with different intervention factors for 72 h. (A) The protein expression of α-SMA and E-cadherin was analyzed using western blot analysis. (B) The protein expression of α-SMA and E-cadherin were quantified using densitometry. (C) The levels of Col I and FN protein in the EEC culture supernatant detected using ELISA. (D) Col I, FN, α-SMA and E-cadherin mRNA expression analyzed using quantitative polymerase chain reaction. The groups were as follows: Control, EECs incubated with serum-free DMEM/F12; Ang II, EECs incubated with 10-6 mol/l Ang II and serum-free DMEM/F12; Ang-(1–7), EECs incubated with 10-5 mol/l Ang-(1–7) and serum-free DMEM/F12; Ang II+Ang-(1–7), EECs incubated with 10-6 mol/l Ang II and 10−5 mol/l Ang-(1–7) and serum-free DMEM/F12. Values are presented as the mean ± standard deviation (n=6). *P<0.05 vs. the control group and #P<0.05 vs. the Ang II group. α-SMA, α-smooth muscle; EEC, endometrial epithelium cell; Ang, angiotensin; col I, collagen type I; FN, fibronectin.