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. 2014 Mar 24;9(6):2138–2144. doi: 10.3892/mmr.2014.2067

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Copyright © 2014, Spandidos Publications

This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.

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Genomic DNA methylation levels analyzed by high-performance liquid chromatography. (A) Sample chromatogram map. The peak time of sample dC was 7.942 min and that of 5mdC was 10.025 min. Nucleotides: A, adenine; T, thymine; G, guanine; dC, deoxidized cytosine; 5mdC, deoxidized 5-methyl cytosine. (B) Quantified genomic DNA methylation levels in the different groups. The genomic DNA methylation rate was 5.23±0.09% in the DN group, 4.71±0.03% in the NDN group and 4.37±0.01% in the NDM group. There were no significant differences among the groups. NDM, non-diabetes control; NDN, diabetes without nephropathy; DN, diabetes with nephropathy; VWD, variable wavelength detector.