Fig. 1.

Protein expression in tissues of Doc2b transgenic mice. (a) Gastrocnemius skeletal muscle (Musc), pancreas (Panc) and epigonadal fat were isolated from three to five pairs of Doc2b Tg mice (black bars) and Wt littermates (white bars) and immunoblotted (IB) for detection of SNARE and SNARE accessory proteins. Doc2b abundances were normalised to clathrin to account for minor variations in protein loading; *p<0.05 vs Wt. (b) Isolated islets were assessed for Doc2b and SNARE protein expression as described in (a) above. (c, d) Heart, liver and spleen were similarly assessed for Doc2b levels (c), as was whole-brain lysate, cerebellum (Cere) and hypothalamus (Hypo) (d). (e) GLUT4 protein abundance was assessed in heart, skeletal muscle and fat from mice from panel (a). Data are representative of three to five pairs of mice. AU, arbitrary units