Figure 9.
The effect of 13-acetoxyrolandrolide on the cell cycle progression in HT-29 cells. Cells were seeded on 10 cm plates, treated with 13-acetoxyrolandrolide (10 μM) for 24 h, and stained with PI (1.0 mg/mL) for analysis using BD FACSCanto. (A) A negative control of HT-29 cells shows 55% of cells in the G1 phase. (B) Treatment with 13-acetoxyrolandrolide showing 68% of HT-29 cells in the G1 phase.