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. Author manuscript; available in PMC: 2015 Jun 24.
Published in final edited form as: Neurosci Lett. 2014 May 10;573:40–45. doi: 10.1016/j.neulet.2014.05.005

Figure 3. Edaravone decreased hypoxia-induced MMP-9 expression in cultured rat OPCs A-B.

Figure 3

Representative images and quantitative data for MMP-9 western blotting using in vitro OPC samples. CoCl2 was used to induce chemical hypoxic stress. HIF-1α western blotting was used as an indicator for hypoxic conditions. β-actin was used as an internal control. Prolonged sub-lethal CoCl2 treatment (1 μM for 7 days) increased MMP-9 expression in OPC cultures, and edaravone (10 μM for 7 days) ameliorated the change. Values are mean ± SD. N=5. *P<0.05 vs CoCl2 group. C-D. LDH and WST assays confirmed that the CoCl2 exposure (1 μM for 7 days) did not induce overt cell death in OPC cultures. Values are mean ± SD. N=5. Eda: edaravone.