Silver-stain and immunoblot of nAra h 2, nAra h 6, and peanut extract using pooled sera from six peanut-allergic patients. A) Silver-stained gel; B) Proteins transferred onto a PVDF membrane were probed with sera, and bound IgE was detected with a peroxidase-labeled goat anti-human IgE. Lane M: Marker, Lane 1: Peanut extract, Lane 2 and 3: nAra h 2, Lane 4: nAra h 6 and Lane 5: T-Ara h 6.