Table 1. Number and percentage of positive test results in parasitology, serology and PCR, according to the status of the participants.

		All suspects at inclusion (N = 237)		Non-confirmed suspects (N = 94)		Parasitologically confirmed cases (N = 143)
		N positive	% positive	N positive	% positive	N positive	% positive
Any parasitological technique		143	60.3	-	-	143	100
Lymph node aspirate†		56	23.6	-	-	56	39.2
Capillary tube centrifugation‡		66	28.5	-	-	66	47.5
mAECT-WB		115	48.5	-	-	115	80.4
mAECT-BC		130	54.9	-	-	130	90.9
CSF examination¥ in CCC or MSC		26	11.0	-	-	26	18.2
Any PCR		224	94.5	87	92.6	137	95.8
PCR - filter paper
INRB	duplicate 1	92	38.8	11	11.7	81	56.6
	duplicate 2	157	66.2	47	50.0	110	76.9
ITM	duplicate 1	147	62.0	32	34.0	115	80.4
	duplicate 2	139	58.7	30	31.9	109	76.2
PCR - GE-buffer
INRB	duplicate 1	146	61.6	21	22.3	125	87.4
	duplicate 2	172	72.6	55	58.5	117	81.8
ITM	duplicate 1	147	62.0	22	23.4	125	87.4
	duplicate 2	146	61.6	18	19.2	128	89.5
Serology
CATT blood titration end titer (≥4)		214	90.3	75	79.8	139	97.2
Trypanolysis (LiTat 1.3)		173	73.0	38	40.4	135	94.4

^†Not done in 128 cases as LNA cannot be applied on suspects without swollen lymph nodes (included in the analysis as not positive).

^‡Not done in 5 cases (result missing in the analysis).

CTC can be done on every suspect, therefore, for the denominator the number of suspects examined was taken, assuming that the positivity rate in the 5 missing suspects is not different from the positivity rate in the examined suspects.

^¥Not done in 84 cases as lumbar puncture, for ethical reasons, is not done on all suspects (included in the analysis as not positive).

CCC = cell counting chamber, MSC = modified single centrifugation; mAECT = mini Anion Exchange Centrifugation Technique, BC = buffy coat, WB = whole blood, CSF = cerebrospinal fluid, GE-buffer = guanidine-hydrochloride EDTA buffer.