Table 3. Tabulation of PCR results. Distribution of participants grouped in function of increasing sum of PCR positive results obtained with their sample in the 8 PCR tests that were performed and proportion of parasitologically confirmed cases in each group.
Sum PCR pos | Filter paper | GE-buffer | ||||||||
INRB | ITM | INRB | ITM | |||||||
Participants | A | B | A | B | A | B | A | B | Paras | |
0 | – | – | – | – | – | – | – | – | 1 (7.7%) | |
1 | 0 | 7 | 0 | 1 | 3 | 8 | 1 | 1 | 3 (14.3%) | |
2 | 13 | 2 | 18 | 6 | 2 | 2 | 19 | 2 | 5 | 5 (17.9%) |
3 | 21 | 3 | 14 | 9 | 13 | 7 | 19 | 5 | 5 | 7 (28.0%) |
4 | 28 | 3 | 8 | 10 | 6 | 6 | 11 | 8 | 8 | 8 (53.3%) |
5 | 25 | 6 | 14 | 10 | 10 | 13 | 15 | 16 | 11 | 9 (47.4%) |
6 | 15 | 5 | 9 | 24 | 24 | 26 | 21 | 26 | 27 | 24 (88.9%) |
7 | 19 | 19 | 33 | 34 | 29 | 35 | 25 | 35 | 35 | 33 (94.3%) |
8 | 27 | 54 | 54 | 54 | 54 | 54 | 54 | 54 | 54 | 53 (98.1%) |
Sum PCR pos: Sum of positive PCR tests per sample; GE-buffer: guanidine hydrochloride EDTA buffer; INRB = Institut National de Recherche Biomédicale; ITM = Institute of Tropical Medicine Antwerp; A and B = replicates A and B on the same sample; Participants: number of participants per group; Paras = parasitologically confirmed sample.