Figure 7. NEU3-HA-GFP triggers phosphorylation of ERK1/2 and Akt.

OFF HeLa NEU3-HA-GFP cells were plated and switched ON for the indicated time periods. 24 h before treatment cells were grown in absence of serum. Before harvesting, cells were stimulated or not for 10 min with EGF. Cell extracts were analyzed for pERK/ERK and pAkt/Akt by western blot using specific primary antibodies. pERK and pAkt optical densities were normalized to ERK and Akt, respectively, and values are given.