Figure 10. Modulation of PKC activity in adult S. mansoni induces sustained coiling.

Adult worm pairs were incubated with increasing concentrations of GF109203X, or 1 µM PMA and movies captured at (B) 1 h, (C) 24 h, (D) 48 h, (E) 72 h, and (F) 96 h and imported into Image J. Movies were then analyzed to determine the number of coils (shown in A) present per worm that were sustained for at least 10 s. Values shown represent the mean (± SEM) percentage of worms displaying a given number of coils at each time point for each treatment. *p≤0.05, **p≤0.01, and ***p≤0.001 (ANOVA).