Figure 1.

Far- and near-UV CD spectra of (a) Rituximab and (b) Etanercept products under (1) native, (2) denaturing, and (3) denaturing-reducing conditions. Dotted lines represent Mabthera (Rituximab) and Enbrel (Etanercept), while continuous lines represent Kikuzubam (Rituximab) and Infinitam (Etanercept) representative batches. Main plot shows near-UV CD spectra (240–350 nm) and graphic insert shows far-UV CD spectra (190–300 nm). Near- and Far-UV characteristic spectra of Rituximab are shown in (a) and insert shows antiparallel β-sheets coming from CH₁, CH₂, and CH₃ and V_L and V_H domains. Etanercept spectra (b) show an irregular structure directed probably by complex and diverse glycan substituents. Each sample was run in triplicate; average is represented as single spectrum (details are described in Section 2.3.6).