Table 5.
DyLight Maleimide method and nonspecific interactions tests under native conditions.
| Protein | 1 | 2 | 3 | 4 |
|---|---|---|---|---|
| Thiol quantification by DLM method | Nonspecific interaction (DLM neutralization) | Protein amine neutralization (protein isothiocyanate treatment) | Thiol quantification by DTNB Method | |
| A Rituximab | 0.0632 ± 0.0274 | 0.0230 ± 0.0001 | 0.0426 ± 0.0094 | 0.0180 ± 0.0032 |
| B Etanercept | 0.0965 ± 0.0028 | 0.0349 ± 0.0000 | 0.0229 ± 0.0000 | 0.0240 ± 0.0049 |
| C Glatiramer acetate | 0.0450 ± 0.0000 | 0.0001 ± 0.0001 | 0.0026 ± 0.0000 | 0.0010 ± 0.0024 |