Figure 2. Agrobacterium-mediated UidA gene transformation in finger millet.

Initially calli were infected with Agrobacterium harboring UidA gene construct and co-cultivated for two days and transferred to the hygromycin selection medium. Photograph of survived calli on selection medium was taken after 30 days (A). Plants (T₀) obtained from the survived calli were analyzed for the presence of UidA gene by PCR (B) and for HptII gene by genomic dot blot (C). Also, the copy number of UidA gene was assessed by Southern blot (D). GUS histochemical assay was performed in transformed calli (T₀ generation; upper panel), T₀ generation finger-let from ear head (UidA-1 T₀; lower panel) (E) and 3 day old T₁ generation seedlings (UidA-1) and the GUS expression were photographed (F). UidA-1 and UidA-2 indicate two independent transgenic lines.