Figure 3. Molecular characterization of finger millet transgenic plants expressing mtlD gene.

Putative finger millet transgenic lines (T₀ generation) transformed with pCAMBIA 1380-mtlD construct were analyzed for the presence of mtlD gene by PCR (A) and HptII gene by dot blot analysis (B). Also, Southern blot was performed to assess the copy number of mtlD gene in these transgenic plants (C). Transcript expression of transgene was analyzed in transgenic plants by semi quantitative RT-PCR. Quantity one (Biorad) software was used to measure the band intensity. The corresponding increase in the relative density of bands over wild-type is presented in the histogram (D).