Figure 6. Performance of transgenic finger millet seedlings expressing mtlD gene under osmotic, salinity and oxidative stress.

Finger millet transgenic (T₂ generation) and wild-type seedlings (1.5 cm length) were initially acclimated with lower concentration of corresponding stress (−0.2 MPa PEG, 50 mM NaCl, and 1 mM menadione) for 8 h and further subjected to indicated concentrations of respective severe stress levels for 48 h. Seedlings were allowed to recover for two days and recovery growth after osmotic stress (A), salinity stress (B) and menadione induced oxidative stress (C) were measured and percent reduction in growth over corresponding control was calculated. Each bar represents the mean of standard error values (n = 20). Experiments were repeated twice. Result from one experiment is presented here and results for another independent experiment were similar. Alphabets above bar indicates the statistical significance (ANNOVA). Same alphabets indicate no significant difference (p<0.05).