Figure 6. Signaling protein expression for WT HL-60 and R38+ and R38− RA-resistant HL-60 cells.

Individual Western blots of whole cell lysates are representative of at least three repeats. GAPDH loading controls were also performed on each individual blot to ensure even loading (not shown). WT HL-60 samples are indicated by WT, R38+ samples are indicated by + and R38− samples are indicated by ---. (A) 24 h protein expression after treatment with a single inducer (RA or D₃) during the precommitment phase. (B) 48 h protein expression after sequential treatment with two inducing agents during the precommitment and lineage-commitment phases (RA/RA, RA/D₃, RA/-, D₃/D₃, D₃/RA, and D₃/-). Quantified blot data are presented in Figure 7.