Figure 3. Traxoprodil (B, C), arcaine (D, E) or DFMO (F, G) rescues dendritic spine number and morphology changes induced by Aβ25–35 in hippocampal neuron cultures.
(A) Representative micrographs depicting GFP-filled dendrites. Cells were incubated with Aβ25–35 for 24 hours and traxoprodil (4 nM), arcaine (4 µM) or DFMO (5 µM) were added 2 hours prior fixation. Spine density and morphology analysis was performed in 20 µm dendrite segments. Spines were classified according to their morphology. Scale bar represent 5 µm. In each experiment 30 to 48 dendritic segments were analyzed Data shown as mean + S.E.M. *P<0.05, **P<0.001, ***P<0.0001 compared to control group or as depicted in graphs.