Figure 4. Abnormal morphology of ambra1 knockdown embryos, as revealed by toluidine blue staining.

Representative longitudinal and cross sections of 3 dpf control and ambra1 morphant embryos. Muscles of ambra1 ATG-morphants show a severe phenotype, with misaligned myofibers scattered in the somitic compartment. Black arrows, areas devoid of staining; white arrows, large myonuclei with condensed chromatin; black arrowheads, interruption of myoseptum; asterisks, opaque material replacing lost myofibers.