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. 2014 Jun 12;9(6):e99732. doi: 10.1371/journal.pone.0099732

Figure 1. Branches of octopaminergic neurons superimpose those of insulin producing cells (IPCs).

Figure 1

A–C Dissected adult brains were stained with rabbit anti-DILP2 (magenta) and octopaminergic neurons were visualized by Tdc2-Gal4 driving GFP (green). Cb, cell bodies of IPCs; Dendr1 and dendr2, presumed dendrites of IPCs. Scale bar = 20 µm. A1–C1 Magnification of regions of interest in A–C. D and E The efficiencies of the UAS-5-HT1A- and UAS-OAMB-RNAi (UAS-OAMB-RNAi GD) were determined by using the pan-neuronal Elav-Gal4 driver and monitoring extracted RNA by qPCR. The transcript value for Elav-Gal4>w1118 was set as 1 in both assays. A significant knockdown (about 60–70% reduction) of 5-HT1A and OAMB transcript was seen (***p<0.001 to both parental controls, One-way ANOVA, n = 2 samples for each genotype).