Skip to main content
. 2014 Jun 12;9(6):e99732. doi: 10.1371/journal.pone.0099732

Figure 4. Knockdown of OAMB or 5-HT1A in IPCs resulted in opposite phenotypes in a capillary feeding assay (CAFE).

Figure 4

A Diminishing 5-HT1A in IPCs (Dilp2-Gal4>5-HT1A-RNAi) significantly decreases food intake only during day 2. The units in the Y-axis are mm food consumed from the calibrated capillary tube (total volume 5 µL; see methods). The graph shows cumulative intake of food over 4 days (*p<0.05, n = 5-10 flies for each genotype in 3 replicates, One-way ANOVA). B Reduction of OAMB level in IPCs (Dilp2-Gal4>OAMB-RNAi) significantly increases food intake from the second day onwards (**p<0.01, ***p<0.001, n = 5–10 flies for each genotype in 3 replicates, One-way ANOVA).