Figure 6. Social behaviors are affected by manipulations of 5-HT_1A and OAMB.

A Aggressive interactions in controls, 5-HT1A-RNAi and OAMB-RNAi knockdown males were determined. All males were between 5–7 days old. The types of behaviors were distributed into three categories, high intensity fighting (HIF), low intensity fighting (LIF) and courtship behavior (Courtship) and the percentage of each type is represented. In all instances the assay was repeated at least 10 times. (n = 20 males/treatment; * P<0.05, ** P<0.005 compared with controls, two-way ANOVA with Bonferroni post hoc test for multiple comparisons). B The percentage of time a male spent actively courting a 3–4 day old wild-type virgin female was determined for control, 5-HT1A-RNAi and OAMB-RNAi knockdown males. C The amount of time, in seconds, before the first courtship behavior, or latency, was determined for control, 5-HT1A-RNAi and OAMB-RNAi knockdown males introduced to 3–4 day old wild-type virgin females. In B and C n = 10 males per genotype; * P<0.05 ** P<0.005 compared with controls (one-way ANOVA with Bonferroni post hoc test for multiple comparisons).