Figure 3. Activation of ASIC1a modifies the current kinetics of GABA_A current.

A, The representative current traces recorded from HEK293 cells co-transfected with GABA_A receptor subunits (α₁ and β₂) and ASIC1a. ASIC1a activated by pH 6 reversibly altered the overall shape of GABA_A currents. GABA_A current traces were superimposed to the right. Red arrow indicates the current activated by pH 6 solution. B, pH 6 solution changed the peak current amplitude but not the shape of GABA_A currents in HEK293 cells transfected with cDNA of GABA_A receptor subunits only. C (cotransfected with both plasmids) and D (transfected with cDNA of GABA_A receptor subunits), bar graph showing the summarized data of rise time of activation (10–90%) (i), desensitization time constant (ii) and deactivation time (iii) of GABA_A currents in the presence of pH 7.4 or pH 6. ***, paired t-test, p<0.001, pH6 group vs. control group, n = 12.