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(A) Nanoparticle complexation capacity determined by detecting free siRNA migration in an agarose gel electrophoresis. Free siRNA was used as positive control. Nanoparticles with different N/P ratios were tested. (B) SYBRGold exclusion assay. The complexation capacity of the prepared nanoparticles was analysed at different N/P rations and at two different pHs (n = 3; average ± SD). * p<0.01.
