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. 2014 Jun 12;9(6):e99568. doi: 10.1371/journal.pone.0099568

Figure 5. Effect of IL-1Ra on VEGFs expression by macrophages when co-cultured with cancer cells.

Figure 5

(A) Effect of anakinra on the migration of macrophages co-cultured with N15 or LNM35 cells; *p<0.05, triplicate samples. (B) Effect of anakinra on Groα/CXCL1, ENA-78/CXCL5, and IL-8/CXCL8 expression in N15 and LNM35 cells as determined by ELISA; *p<0.05 and **p<0.01, triplicate cultures. Effect of a CXCR2 antagonist (C) and a CXCR2 neutralizing antibody (D) on macrophage migration induced by highly metastatic LNM35 cells; *p<0.05 and **p<0.01, triplicate cultures. (E) Expression of mouse VEGF-A, VEGF-C, and VEGF-D in macrophages co-cultured directly or indirectly with LNM35 cells. In indirect co-cultures, mouse macrophages were placed in the lower chamber with or without cancer cells in the upper chamber. After 4 days, mouse VEGF-A, VEGF-C, and VEGF-D mRNA expression was analyzed by qRT-PCR; *p<0.05 and **p<0.01, triplicate cultures. (F) Effect of anakinra on mouse VEGF-A, VEGF-C, and VEGF-D expression in macrophages directly co-cultured with LNM35 cells. Mouse macrophages were co-cultured with or without cancer cells for 4 days in the presence or absence of 50 ng anakinra/mL, followed by the analysis of mouse VEGF-A, VEGF-C, and VEGF-D mRNA expression by qRT-PCR; **p<0.01, triplicate cultures.