Skip to main content
. 2014 Jun 12;10(6):e1004180. doi: 10.1371/journal.ppat.1004180

Figure 2. SB505124 induces bradyzoite development.

Figure 2

A. HFFs growing in 24 well plates were infected with RH, GT1, Pru, and CTG. After 5–7 days, monolayers were methanol-fixed, stained with crystal violet, and plaques were counted. Means and standard deviations are shown. B. HFFs grown on glass coverslips were infected with Pru strain parasites and either mock-treated or treated with pH 8.2 media or 3 µM SB505124. After 72 h, cells were fixed and stained with anti-SAG1 antisera and Dolichos-FITC to visualize cysts. Shown are representative images. C. HFFs were infected with Pru and either mock-treated or treated with pH 8.2 media or 3 µM SB505124. After 72 h, parasites were released from host cells, washed, and lysed. Total RNA was extracted, DNase I-treated and converted to cDNA. Relative amounts of transcripts were determined by Real Time PCR using β-actin as an internal reference. Fold change for each gene's transcript abundance is reported as 2−ΔΔCt. Shown are averaged data with standard deviations from 3 experiments. Note that changes in ENO2 are not statistically significant.