Figure 5.
High performance liquid chromatography analysis of the intracellular concentration of 5-FU. The concentration of 5-FU was calculated as the percentage of the total area under the curve for each sample. Intracellular concentrations of 5-FU in untreated control cells were 0.50±0.17 and 0.28±0.11 for Bel-7402 and Bel-7402/FU cells, respectively. Data represent the mean ± standard deviation of three independent experiments. **P<0.01, compared with Bel-7402/FU cells treated with 0.025 mg/ml 5-FU. ASIV, astragalocide IV; 5-FU, 5-fluorouracil.