Table 2. Spore-killing activity of selected het-s mutants in a cross a with het-S male parent.
maternal parent in cross | 2-spored asci/total counted asci | spore-killing (%) | p-value (significance of difference to wt)* |
het-S | 0/272 | 0 | <0.0001 |
het-s wt | 445/632 | 70.4 | - |
N226A | 7/664 | 1 | <0.0001 |
Q240A | 0/425 | 0 | <0.0001 |
E272A | 328/746 | 44 | <0.0001 |
K284A | 31/506 | 5.8 | <0.0001 |
D288A | 190/509 | 37.3 | <0.0001 |
T233A/S273A | 311/836 | 37.2 | <0.0001 |
E234A/R236A/E265A | 94/654 | 14.4 | <0.0001 |
*p-value is calculated in a two-tailed Fisher's test.