Figure 2. Schematic of the overexpression library and the growth conditions analyzed.

After transfection, the library was grown under non-inducing (tet −) and inducing conditions (tet +) with increasing concentration of blasticidin and then, genomic DNA was isolated from survival populations. Adaptor-ligated sequencing libraries were prepared from specific PCR reactions and Illumina sequenced. Illumina sequencing reads containing a backbone vector junction sequence were then mapped to the T. brucei reference.