Table 1. RNA-binding proteins and translation factors that increase BLA resistance.
Locus number | Description | Number locations | Fold per CDS |
RNA binding proteins | |||
Tb927.4.400 | DRBD7 | 3 | 11.5 |
Tb927.10.11270 | RBP23 | 7 | 23.6 |
Tb927.7.880 | RBP25 | 5 | 8.7 |
Tb927.10.13720 | RBP29 | 8 | 10.1 |
Tb927.6.4440 | RBP42 | 15 | 10.6 |
Tb927.9.8740 | DRBD3 (PTB1) | 2 | 14.6 |
Tb927.11.14100 | DRBD4 (PTB2) | 10 | 11.1 |
Tb927.3.3960 | DRBD6A | 7 | 42.8 |
Tb927.3.790 | ZC3H6 | 10 | 4.8 |
Tb927.5.810 | ZC3H11 | 10 | 24.0 |
Tb927.7.2660 | ZC3H20 | 9 | 122.1 |
Tb927.7.2670 | ZC3H21 | 7 | 65.1 |
Tb927.8.4020 | ZC3H24 | 6 | 26.2 |
Tb927.8.4070 | ZC3H25 | 7 | 26.2 |
Tb927.8.4120 | ZC3H26 | 7 | 26.2 |
Tb927.9.9450 | ZC3H28 | 24 | 25.3 |
Tb927.10.5150 | ZC3H31 | 4 | 4.2 |
Tb927.10.12330 | ZC3H34 | 7 | 20.9 |
Tb927.10.12760 | ZC3H36 | 7 | 50.0 |
Tb927.10.12780 | ZC3H37 | 10 | 64.7 |
Tb927.10.12800 | ZC3H38 | 15 | 48.6 |
Tb927.10.14930 | ZC3H39 | 6 | 6.8 |
Tb927.10.14950 | ZC3H40 | 16 | 15.4 |
Tb927.2.4710 | TRRM1 | 5 | 6.0 |
Tb927.9.9290 | PABP1 | 11 | 58.4 |
Tb927.9.10770 | PABP2 | 10 | 45.7 |
Tb927.11.6240 | 2 weak RBDs | 10 | 14.1 |
Tb927.3.1910 | histone RBD | 4 | 6.2 |
Other known factor | |||
Tb927.8.4540 | PBP1 | 15 | 91.6 |
Translation factors | |||
Tb927.7.1710 | DOM34 | 2 | 14.3 |
Tb927.3.2900 | EF-2 alpha | 6 | 6.7 |
Tb927.11.11770 | eIF4E3 | 5 | 88.6 |
Tb927.6.1870 | eIF4E4 | 18 | 23.3 |
Tb927.9.5460 | eIF4G2 | 10 | 5.1 |
Tb927.11.10560 | eIF4G4 | 8 | 38.5 |
Tb927.8.4500 | eIF4G5 | 9 | 26.5 |
Tb927.11.5840 | SUI1 homologue | 2 | 20.6 |
Tb927.11.12670 | TYW3 homologue | 4 | 14.2 |
The number of locations is the number of positions at which a fusion protein caused at least a 3-fold increase in reads per million in the BLA6x or BLA10x condition. ‘Fold per CDS’ is the average increase for the whole CDS, including experiment A (BLA6x, 30 µg/ml) and experiment B (BLA10x, 50 µg/ml). Other proteins that are thought to increase stability and/or translation were positive in only one experiment or with only one fragment. These included the pumilio domain protein PUF9 [12], the ALBA proteins [13], the small CCCH protein ZFP3 [9], [58], [59] the 33 kDa subunit homolog of the cycle sequence binding protein (CSBP, [60] and RBP33 [34].