Table 1.
List of primers and taqman probes used for semi-quantitative RT-PCR
| Primer | Accession numbers | Primer sequence | Product lenght (bp) |
|---|---|---|---|
| GAPDH (forward) |
AB028142 |
5′-GCT GCCAAATAT GACGACATC A-3′ |
75bp |
| GAPDH (reverse) |
|
5′-GTA GCC CAG GAT GCC TTT GAG-3′ |
|
| GAPDH (TaqMan Probe) |
|
5′-TCC CTC CGA TGC CTG CTT CAC TAC CTT-3′ |
|
| 18SrRNA (forward) |
FJ797658 |
5′-GTC GCT CGC TCC TCT CCT ACT-3′ |
125bp |
| 18SrRNA (reverse) |
|
5′-GGC TGA CCG GGT TGG TTT-3′ |
|
| 18SrRNA (TaqMan Probe) |
|
5′-ACA TGC CGA CGG GCG CTG AC-3′ |
|
| PGR (forward) |
NM_001003074 |
5′-CGA GTC ATT ACC TCA GAA GAT TTG TTT-3′ |
113bp |
| PGR (reverse) |
|
5′-CTT CCA TTG CCC TTT TAA AGA AGA-3′ |
|
| PGR (TaqMan Probe) |
|
5′-AAG CAT CAG GCT GTC ATT ATG GTG TCC TAA CTT-3′ |
|
| COX2 (forward) |
HQ110882 |
5′-GGA GCA TAA CAG AGT GTG TGA TGT G-3′ |
87bp |
| COX2 (reverse) |
|
5′-AAG TAT TAG CCT GCT CGT CTG GAA T-3′ |
|
| COX2 (TaqMan Probe) |
|
5′-CGC TCA TCA TCC CAT TCT GGG TGC-3′ |
|
| PGFS/AKR1C3 (forward) |
NM_001012344 |
5′-AGG GCT TGC CAA GTC TAT TGG-3′ |
74bp |
| PGFS/AKR1C3 (reverse) |
|
5′-GCC TTG GCT TGC TCA GGA T-3′ |
|
| PGFS/AKR1C3 (TaqMan Probe) |
|
5′-TCC AAC TTT AAC CGC AGG CAG CTG G-3′ |
|
| PGES (forward) |
NM_001122854 |
5′-GTC CTG GCG CTG GTG AGT-3′ |
89bp |
| PGES (reverse) |
|
5′-ATG ACA GCC ACC ACG TAC ATC T-3′ |
|
| PGES (TaqMan Probe) |
|
5′-TCC CAG CCT TCC TGC TCT GCA GC-3′ |
|
| PTGFR (FP) (forward) |
NM_001048097 |
5′-ACC AGT CGA ACA TCC TTT GCA-3′ |
86bp |
| PTGFR (FP) (reverse) |
|
5′-GGC CAT CAC ACT GCC TAG AAA-3′ |
|
| PTGFR (FP) (TaqMan Probe) |
|
5′-CAT GGT GTT CTC CGG TCT GTG CCC-3′ |
|
| PTGER2 (forward) |
AF075602 |
5′- CAC CCT GCT GCT GCT TCT C-3′ |
78bp |
| PTGER2 (reverse) |
|
5′-CGG TGC ATG CGG ATG AG-3′ |
|
| PTGER2 (TaqMan Probe) |
|
5′-TGC TCG CCT GCA ACT TTC AGC GTC-3′ |
|
| PTGER4 (forward) |
NM_001003054 |
5′-AAA TCA GCA AAA ACC CAG ACT TG-3′ |
96bp |
| PTGER4 (reverse) |
|
5′-GCA CGG TCT TCC GCA GAA -3′ |
|
| PTGER4 (TaqMan Probe) |
|
5′-ATCCGA ATT GCT GCT GTG AAC CCT ATC C-3′ |
|
| PGT (forward) |
NM_001011558 |
5′-TGC AGC ACT AGG AAT GCT GTT C-3′ |
116bp |
| PGT (reverse) |
|
5′-GGG CGC AGA GAA TCA TGG A-3′ |
|
| PGT (TaqMan probe) |
|
5′-TCT GCA AAC CAT TCC CCG CGT G-3′ |
|
| HPGD (forward) |
NM_001284477 |
5′-GGC AGC GAA TCT CAT GAA CAG-3′ |
93bp |
| HPGD (reverse) |
|
5′-TCT TCT TTC TCA ATG GAT TCA AGGA-3′ |
|
| HPGD (TaqMan Probe) | 5′-TGA ATG CCA TTT GCC CAG GCT TTG-3′ |