Fig. 1.

Production of pro-inflammatory cytokines and chemokines from rAd5-EAT2-infected human PBMCs. Human PBMCs were isolated from fresh buffy coat material and were either mock infected or infected with MOI of 10000 with rAd5-EAT2, rAd5-EAT2(R31Q) or rAd5-Null. Culture media were collected at 72h post Ad infection and were analyzed for cytokine and chemokine levels using a 27-plex multiplexed bead array-based quantitative system. Samples were plated in quadruplicates. Data are representative of at least three independent experiments from three different blood donors with similar results. The bars represent mean ± SD. Statistical analysis was completed using a one way ANOVA with a Student–Newman–Keuls post-hoc test. P <0.05 was deemed a statistically significant difference. *P < 0.05, **P < 0.01, ***P < 0.001, statistically significant from mock-treated cells.