Fig. 5.

Monocytes derived from rAd5-EAT2-infected human PBMCs exhibit enhanced phagocytosis capabilities. Human PBMCs were isolated from fresh buffy coat material and were either mock infected or infected with MOI of 10000 with rAd5-EAT2, rAd5-EAT2(R31Q) or rAd5-Null. Cells were then incubated with FITC⁺ latex beads and analyzed by FACS as described in Methods. Data are representative of two independent experiments with similar results. Samples were plated in quadruplicates. Bars represent mean ± SD. Statistical analysis was completed using a one way ANOVA with a Student–Newman–Keuls post-hoc test. P <0.05 was deemed a statistically significant difference. **P < 0.01, statistically significant from mock-treated cells.