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. 2012 Oct 18;208(1):130–138. doi: 10.1093/infdis/jis636

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© The Author 2012. Published by Oxford University Press on behalf of the Infectious Diseases Society of America. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

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Figure 5. — PTX3 localization in sputum specimens from patients with cystic fibrosis (CF). Sputum specimens from 10 CF patients were isolated and characterized for PTX3 localization by indirect immunofluorescence, using a human PTX3 antibody. Texas red–labeled goat anti-rabbit immunoglobulin G was used as secondary antibody; DAPI labeling was used to visualize cell nuclei and extracellular DNA strands. A and B, Localization of PTX3 in cells of the immune system, mainly neutrophils (insets). B, PTX3 entrapped in the actin-DNA filaments (insets). C, PTX3 bound to Pseudomonas aeruginosa macrocolonies (insets). Original magnification: 40 × , for panels A–C; and 100 × , for insets.