Fig. 4.

Effect of peritoneal cavity conditioning on tumor implantation in the abdominal wall. Effect of peritoneal cavity conditioning on the implantation of CT-26 tumor cells (0.3 × 10⁶) injected in mice not subjected to surgery or pneumoperitoneum (group I) or after 60 min of pneumoperitoneum with non-humidified CO₂ (group II; dark bar). To the latter group, we sequentially added beneficial factors such as humidification (group III), 10 % N₂O (group IV), 4 % O₂ (group V), and cooling to a body temperature of 32 °C (group VI). The body temperature was 37 °C in all other groups. Tumor implantation in the abdominal wall increased with the peritoneal damage (p = 0.003) and decreased using humidified CO₂ (p = 0.032) and conditioning (p = 0.026)