Fig. 2.

Analysis of peptide and urine-evoked Ca²⁺ responses in intact VSNs. a Example of somatic Ca²⁺ recordings from a single VSN to different concentrations of SIINFEKL. A response is defined as a stimulus-dependent deviation in Ca²⁺ fluorescence that exceeds twice the standard deviation of the mean of the baseline fluorescence noise. b SIINFEKL dose–response curves (grey) each recorded from a single VSN (n = 12 cells, eight slices). Relative changes in peak fluorescence (ΔF/F) induced by a given stimulus were normalized to the maximum peak response measured in a given VSN to construct a dose–response curve. The black curve represents the mean dose–response curve; EC₅₀ value and SD are indicated. c Comparison of Ca²⁺ responses in a single VSN to various concentrations of SIINFEKL and urine (DU, diluted 100-fold to simulate the decrease in concentration in the VNO duct) obtained from B6/OVA⁺, B6/OVA⁺/β₂m^−/−, and B6 mice. This figure is taken from Sturm et al. [34], where further details can be found. Reprinted by kind permission of Trese Leinders-Zufall