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. 2014 Jun 13;4:4805. doi: 10.1038/srep04805

Figure 3. DJ-1 regulates the expression of NF-κB target cytokines.

Figure 3

(a) The expression of pro-inflammatory cytokines, including IL-1β, IL-6 and MCP-1 at 6 d after adipogenesis induction in 3T3-L1 cells. *, p < 0.05 and **, p < 0.01 versus the control. (b) The secretion of IL-6 in anti-DJ-1 siRNA-treated 3T3-L1 cells was determined with a multiplex mouse adipokine assay. The IL-6 concentration in 3T3-L1 cells was examined from 0 d to 6 d after adipogenesis induction. *, p < 0.05 and **, p < 0.01 versus the control. (c) NF-κB activity was examined using the luciferase reporter gene assay in RAW264.7 cells in the absence or presence of LPS (100 ng/ml). Knock-down cells were incubated with LPS for 8 h, and luciferase activity was determined. *, p < 0.05 versus the control. #, p < 0.05 versus the vehicle. (d) The concentration of nitrate was detected in non-targeting or anti-DJ-1 siRNA-treated cells. After RAW264.7 cells were transfected with siRNA, the cells were treated with LPS (100 ng/ml) for 8 h. *, p < 0.05 versus the control. #, p < 0.05 versus the vehicle. All data are presented as the mean ± s.d.