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. 2014 Jun 13;4:5281. doi: 10.1038/srep05281

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(a) Schematic for selective coating procedure of kinesin and dynein. (1) Biotinylated SAM grafting on the gold patterned region. (2) Kinesin, (3) streptavidin, and (4) dynein were immobilized sequentially. Selective coating was examined by injecting (5) PMMT, and the gliding direction was evaluated (6). (b), (c) PMMTs gliding on the selectively immobilized motors in the (b) SAM-coated region (led by dimmer plus end) and (c) glass region (led by brighter minus end). Scale bar, 10 μm. (d), (e) Polarity of PMMTs gliding on the (d) SAM-coated region and (e) glass region. Control surfaces were prepared by omitting kinesin in (d) or dynein in (e) from the optimum assay for the selective coating. In both cases, polarity distribution was not significantly different (p > 0.05) from control experiments as determined with the chi-square test. n.s. = not significant.