Figure 6. Impact of statins on cellular and C. elegans metabolism.
(A–G) Effect of 24 hours treatment with atorvastatin, fluvastatin and simvastatin in Hepa1.6 on ATP level (A), redox potential (B), Δψm (C), DNA content (D), extra-cellular acidification rate (ECAR) (E), basal oxygen consumption rate (OCR) (F) and uncoupled OCR (G). (H) Acute effect of statins on OCR in Hepa1.6. Measurement was performed 3 minutes after addition of the compounds. (I) Fluvastatin does not impair the development of C. elegans N2 worms at 10, 20 and 50 μM. Pictures were taken at day 3 of adulthood. (J–K) Effect of fluvastatin on basal OCR (J) and uncoupled OCR (K) at day 1 of adulthood. (L) Lifespan in N2 worms after treatment with fluvastatin at 20 and 50 μM. Results are expressed as ratio over DMSO control and as mean ± SEM. Grey zone represents the DMSO 95% confidence interval. Significance was tested using a two-way ANOVA test, with Bonferroni correction for multiple comparison. * P<0.05, ** P<0.01 and *** P<0.001.