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. 2014 Jun 3;10:16. doi: 10.1186/1746-4811-10-16

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Copyright © 2014 Kirigia et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Viral induced gene silencing (VIGS) via agro-infiltration method on S. hermonthica. a, b, c, d, and e are plants agro-infiltrated with Agrobacterium strain GV3101 habouring TRV2-PDS (GV3101/TRV2-PDS), and mixed in 1:1 ratio with GV3101 containing TRV1 empty vectors (GV3101/TRV2), in days 1, 7, 14, 21 and 28 after agro-infiltration respectively; f, g, h, i and j are plants agro-infiltrated with GV3101 vectors containing TRV1 empty (GV3101/TRV1) in days 1, 7, 14, 21 and 28 after infiltration respectively; k, l, m, n, and o are plants agro-infiltrated with GV3101 only, in days 1, 7, 14, 21 and 28 after agro-infiltration respectively. All scale bars represent 5 cm.