Figure 4.

F8 mRNA is expressed in selective endothelial cell beds. Plots show fold-change (enrichment or depletion) of mRNA transcripts for a number of genes in the endothelial cell mRNA isolated from (A) liver, (B) kidney, (C) heart, and (D) brain tissues of 4 RiboTag, Tek-Cre⁺ mice relative to the whole-tissue lysate input (set to 1.0). Values <1.0 indicate depletion in the endothelial cell fraction, relative to input. In contrast, values >1.0 indicate enrichment. Each symbol represents an independent animal. Values for tissue-specific, non–endothelial cell genes are shown as open circles. Values for endothelial cell–specific genes are shown as light gray squares. Values for F8 mRNA fold-change are shown as black diamonds. Horizontal lines indicate mean and error bars indicate standard error of the mean for each genotype.